Anaerobic Obligatory Xylitol Production in Escherichia coli Strains Devoid of Native Fermentation Pathways

Anaerobic obligatory xylitol production in Escherichia coli strains devoid of native fermentation pathways.

Anaerobic glucose oxidation was coupled to xylose reduction in a nonfermentative Escherichia coli strain expressing NADPH-dependent xylose reductase. Xylitol production serves as the primary means of NAD(P)(+) regeneration, as glucose is converted primarily to acetate and CO(2). The membrane-bound transhydrogenase PntAB is required to achieve the maximum theoretical yield of four moles of xylit...

Hydrogen production by recombinant Escherichia coli strains

The production of hydrogen via microbial biotechnology is an active field of research. Given its ease of manipulation, the best-studied bacterium Escherichia coli has become a workhorse for enhanced hydrogen production through metabolic engineering, heterologous gene expression, adaptive evolution, and protein engineering. Herein, the utility of E. coli strains to produce hydrogen, via native h...

Fermentation study of metabolically engineered Escherichia coli strains for high-level 1-propanol production

Homolactate fermentation by metabolically engineered Escherichia coli strains.

We report the homofermentative production of lactate in Escherichia coli strains containing mutations in the aceEF, pfl, poxB, and pps genes, which encode the pyruvate dehydrogenase complex, pyruvate formate lyase, pyruvate oxidase, and phosphoenolpyruvate synthase, respectively. The process uses a defined medium and two distinct fermentation phases: aerobic growth to an optical density of abou...

In Vitro Inhibition of Adhesion of Escherichia coli Strains by Xylitol

The present study aimed to evaluate xylitol’s antimicrobial and anti-adherence activities on Escherichia coli (ATCC 8739) and on another clinical strain enteropathogenic E. coli (EPEC). In vitro minimum inhibitory concentration (MIC) test and adhesion assays were performed using 0.5, 2.5 and 5.0% xylitol. It was found that xylitol did not have antimicrobial properties on these strains. The scan...